School of Dentistry
Dental Practice & Rural Health
Objective. A genetic component in early childhood caries (ECC) is theorized, but no genomewide investigations of ECC have been conducted. This pilot study is part of a long-term research program aimed to: (1) determine the proportion of ECC variance attributable to the human genome and (2) identify ECC-associated genetic loci. Methods. The study’s community-based sample comprised 212 children (mean age=39 months; range = 30–52 months; males = 55%; Hispanic/ Latino = 35%, African-American = 32%; American Academy of Pediatric Dentistry definition of ECC prevalence = 38%). Approximately 2.4 million single nucleotide polymorphisms (SNPs) were genotyped using DNA purified from saliva. A P < 5 9 108 criterion was used for genome-wide significance. SNPs with P < 5 9 105 were followed-up in three independent cohorts of 921 preschool-age children with similar ECC prevalence. Results. SNPs with minor allele frequency ≥5% explained 52% (standard error = 54%) of ECC variance (one-sided P = 0.03). Unsurprisingly, given the pilot’s small sample size, no genomewide significant associations were found. An intergenic locus on 4q32 (rs4690994) displayed the strongest association with ECC [P = 2.3 9 106 ; odds ratio (OR) = 3.5; 95% confidence interval (CI) = 2.1–5.9]. Thirteen loci with suggestive associations were followed-up – none showed evidence of association in the replication samples. Conclusion. This study’s findings support a heritable component of ECC and demonstrate the feasibility of conducting genomics studies among preschool-age children.
Digital Commons Citation
Ballantine, Jami L.; Carlson, Jenna C.; Ferreira Zandoná, Andrea G.; Agler, Cary; Zeldin, Leslie P.; Rozier, Richard Gary; Roberts, Michael W.; Basta, Patricia V.; Luo, Jason; Antonio-Obese, Mikafui E.; McNeil, Daniel W.; Weyant, Robert J.; Crout, Richard J.; Slayton, Rebecca L.; Levy, Steven M.; Shaffer, John R.; Marazita, Mary L.; North, Kari E.; and Divaris, Kimon, "Exploring the genomic basis of early childhood caries: a pilot study" (2018). Clinical and Translational Science Institute. 749.
Ballantine JL, Carlson JC, Ferreira Zandoná AG, et al. Exploring the genomic basis of early childhood caries: a pilot study. International Journal of Paediatric Dentistry. 2017;28(2):217-225. doi:10.1111/ipd.12344