Author ORCID Identifier

https://orcid.org/0000-0002-1464-2245

Semester

Spring

Date of Graduation

2023

Document Type

Thesis

Degree Type

MS

College

School of Dentistry

Department

Not Listed

Committee Chair

Samuel Dorn

Committee Co-Chair

Mark Byron

Committee Member

Mark Byron

Committee Member

Dr. Constance Wiener

Abstract

Introduction:

One of the goals of endodontic therapy is to debride infected pulps of teeth and return the tooth to a state of health in preparation of restorative care and return to function. Chemically cleansing of the canal system augments mechanical debridement by flushing out debris, dissolving tissue, and disinfecting the root canal system. The purpose of this research is to compare the efficacy of five irrigants: 0.008% HOCl acid (Endocyn®), 0.03% HOCl (Vashe®), 6% and 8.25% sodium hypochlorite (NaOCl), and Ozone against a typical bacterial species found in infected pulps, Enterococcus faecalis (E. faecalis).

Methods:

After 24 hours of growth of E. faecalis onto an Innovatech 96 well hydroxyapatite coated peg plate, the biofilms were introduced to five different irrigants for 30 seconds of exposure, then immersed into a Difco D/E Neutralizing broth solution for 30 minutes. Each hydroxyapatite peg was placed into 1.5mL centrifuge tubes and diluted by a factor of 10. Dilution samples were transferred into experimental 5mL bottom round test tubes and the Invitrogen’s Molecular Probes LIVE/DEAD BacLight Bacterial Viability Counting Kit was used to prepare the final samples for analysis at WVU’s Flow Cytometry and Single Cell Core Facility.

Results:

There was a significant difference in antibacterial effectiveness among the five irrigant groups (p

Conclusions:

All irrigants tested had antibacterial properties against E. faecalis. 6% NaOCl and 8.25% NaOCl demonstrated the greatest antibacterial effect against E. faecalis compared to other irrigants.

Comments

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