Date of Graduation
School of Dentistry
John G. Thomas.
The mutagenic activities of the root canal sealers, RSA Roekoseal Automix and AHPIus, were tested in the bacterial gene mutation assay (Ames test). Both materials were tested immediately after mixing and after setting 24 h. The Salmonella typhimurium tester strains TA98 and TA 100 were used to detect the induction of frame shift mutations and base pair substitutions both in the presence and absence of a metabolically active microsomal fraction from rat liver (S9 fraction). No mutagenic or toxic effects were found with DMSO eluates of freshly mixed or 24h set RSA Roekoseal Automix. However, under extreme experimental conditions, DMSO eluates of the freshly mixed and 24 hr set AHPlus were mutagenic in both TA98 and TA100 in a dose related manner. AHPIus was toxic to TA100 at the highest concentration. Therefore, RSA Roekoseal Automix is nonmutagenic in the Ames test to tester strains TA98 and TA100 and less mutagenic than AHPlus.
Wateska, Joseph Anthony, "Mutagenicity of root canal sealer RSA Roekoseal Automix in the Ames test" (2001). Graduate Theses, Dissertations, and Problem Reports. 1277.