Date of Graduation
School of Medicine
Physiology, Pharmacology & Neuroscience
Donald A. Sens
The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to Cd2+ following recovery in normal medium showed the expression of MT-1A, MT-1F, MT-1G genes and a 3 fold induction of MT-1E and a lesser induction of MT-1X and MT-2A. The basal level of MT-1/2 protein was found to be 3.2 +/- 0.17 ng MT/total protein with levels gradually increasing as a result of Cd2+ exposure. No MT-3 protein was induced on exposure to Cd2+.
Neriyanuri, Madhuri G., "Characterization of metallothionein expression in response to cadmium in the 267-B1, normal prostate cell line" (2002). Graduate Theses, Dissertations, and Problem Reports. 1561.