Date of Graduation
School of Pharmacy
Robert L. Haining.
To date, knowledge in characterization of CYP proteins has been limited to immunoblotting, RT-PCR, immunohistochemistry and so forth; therefore, it is the intention of this study to investigate: (1) What kind of CYP2D protein is present in brain with modern proteomic tools; (2) Whether, CYP2D6 in brain is same as the liver CYP2D6; and (3) If CYP2D7 is present, can CYP2D6 polymorphism be found in the brain?;To answer these questions, this study used a one-step method of isolation of protein by immunoprecipitation followed by its identification using 2D-blots, nanospray LCMS, immunoblots and immunohistochemistry. Probing of immunoprecipitated proteins with polyclonal CYP2D6 antibody revealed two major CYP2D6 immunoreactive bands. Similar banding pattern resulted from matched human liver microsomes suggesting there are more than one CYP2D isoform(s) exist in these tissues.
Bhatia, Deepak, "Characterization of CYP2D protein from human brain cerebellum" (2004). Graduate Theses, Dissertations, and Problem Reports. 2019.