Date of Graduation


Document Type


Degree Type



School of Dentistry


Not Listed

Committee Chair

Michael Bagby.


A new endodontic root canal sealer, iRoot SP, has been introduced. This bioceramic root canal sealer claims to be a superior sealing and filling material for root canals. Although the manufacturer advocates the use of a single cone technique with this sealer, they state that it may also be used with lateral compaction and warm vertical condensation. The purpose of this study was to compare in vitro apical microleakage of iRoot SP root canal sealer to AH Plus sealer when used in conjunction with a single cone technique and lateral compaction technique.;Eighty six mesial roots of mandibular molar teeth with mature apices were randomly divided into four experimental groups along with a positive and negative control groups. Teeth were cleaned and sectioned at the CEJ with high speed hand piece and water coolant. All teeth were pre-flared with S1 and S2 Protaper Universal (DENTSPLY RTM) NiTi rotary files. Then the teeth were instrumented to a #30 .06 taper master apical file with Brasseler RTM Endosequence Niti rotary files in a crown down technique. Teeth were then divided into the groups: Group A- lateral condensation technique with iRoot Sp, Groups B- lateral condensation with AH Plus, Group C- single cone technique with iRoot SP, Group D- single cone with AH Plus, positive control (obturation with no sealer), and negative control (no obturation with two layers of varnish covering entire root). Teeth were allowed to soak in 1 % methylene blue dye under vacuum (390 torr) and for an additional 7 days once the seal was broken. The clearing process consisted of 24 hours in 5% nitric acid, 24 hours in 80% ethyl alcohol, 24 hours in 99.8% ethyl alcohol, and the roots became transparent after 48 hours in 99 % methyl salicylate. Two second year endodontic residents along with a dental hygienist graded the apical dye penetration a measuring microscope.;None of the negative controls showed any leakage and all of the positive control showed leakage the entire length of the root. Of the 86 specimens starting the study, 4 did not clear properly and were excluded from the study leaving a total of 82. In the iRoot SP group, the mean apical leakage in group A was 0.83mm +/- 0.15, and 0.63mm +/- 0.13 for group C. In the AH Plus group, the mean apical leakage was 1.63mm +/- 0.13 in group B, and 0.42mm +/- 0.14 in group D.;Comparable levels of apical leakage were found between iRoot SP (single cone and lateral compaction) and AH plus single cone. AH plus with lateral compaction had statistically more apical dye leakage that the other groups. None of the negative controls showed any leakage and all of the positive controls demonstrated leakage supporting the experimental design. This in vitro study supports the use of the sealers AH Plus or iRoot SP in combination with .06 taper gutta-percha points in the obturation of .06 canal preparations.