Date of Graduation
Eberly College of Arts and Sciences
We have examined the effects of the cytokines tumor necrosis factoralpha (TNF), transforming growth factorbeta (TGFbeta) and the effect of cyclooxygenase inhibitor ibuprofen on prostaglandin and 15-HETE biosynthesis by normal adult human osteoblast-like (hOB) cells. In the present studies, conditioned media harvested from hOB cell cultures were assayed by eicosanoid specific EIAs to determine quantitatively the effects of cytokines on PGE2, 6-keto-PGF1alpha, PGF2alpha, PGD2, thromboxane (TXB2) and on 15-HETE biosynthesis. EIA analysis indicated that PGE2 was the predominant PG metabolite released by unstimulated hOB cells and its synthesis was increased more than other PGs in cells treated with (TGFbeta) and TNFalpha, both separately and in combination.;Thus, these studies indicate that (TGFbeta) and/or TNFalpha, modulate AA metabolism and upregulation of PGE2 synthesis elicited by these cytokines might have resulted from an increased level of the terminal PGE 2 synthases in hOB cells. (Abstract shortened by UMI.).
Grover, Anurag, "Osteotropic cytokines mediate human osteoblast-like cell eicosanoid production" (2005). Graduate Theses, Dissertations, and Problem Reports. 4151.