Semester
Spring
Date of Graduation
2013
Document Type
Dissertation
Degree Type
PhD
College
Eberly College of Arts and Sciences
Department
Chemistry
Committee Chair
Lisa Holland
Committee Co-Chair
Parviz Famouri
Committee Member
Fred King
Committee Member
Michelle Richards-Babb
Committee Member
Ronald Smart.
Abstract
Aqueous phospholipid preparations comprised of 1,2-dimyristoyl- sn-glycero-3- phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) are prevalent materials for biological characterization. The viscosity behavior of DMPC/DHPC was characterized as a function of q value (q= [DMPC]/[DHPC] ), hydration as well as temperature firstly on bench-top and then in microfluidic chip. The rheology of 20% phospholipid preparations of [DMPC]/[DHPC] = 2.5 reveals that, under conditions utilized for fluid steering, the materials are shear-thinning power-law fluids with a power-law index ranging from 0.30 through 0.90. Phospholipid preparations are utilized to steer fluids in microfluidic chips and support hydrodynamic delivery of sample across a double T injection region in a chip. The fact that the phospholipids are fully integrated as a valving material as well as a separation medium is demonstrated through the separation of linear oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid.
Recommended Citation
Wu, Xingwei, "Characterization and Application of Self-Assembled Nano-Materials for Fluid Steering and Separation in Microfluidics" (2013). Graduate Theses, Dissertations, and Problem Reports. 5013.
https://researchrepository.wvu.edu/etd/5013