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School of Pharmacy


Pharmaceutical Sciences


Synthetic amorphous silica nanoparticles (SAS NPs) have been used in various industries, such as plas-tics, glass, paints, electronics, synthetic rubber, in pharmaceutical drug tablets, and a as food additive in many processed foods. There are few studies in the literature on NPs using gene mutation approaches in mammalian cells, which represents an important gap for genotoxic risk estimations. To fill this gap, themouse lymphoma L5178Y/Tk+/−assay (MLA) was used to evaluate the mutagenic effect for five different concentrations (from 0.01 to 150 g/mL) of two different sizes of SAS NPs (7.172 and 7.652 nm) and a fine collodial form of silicon dioxide (SiO2). This assay detects a broad spectrum of mutational events, from point mutations to chromosome alterations. The results obtained indicate that the two selectedSAS NPs are mutagenic in the MLA assay, showing a concentration-dependent effect. The relative muta-genic potencies according to the induced mutant frequency (IMF) are as follows: SAS NPs (7.172 nm)(IMF = 705.5 × 10−6), SAS NPs (7.652 nm) (IMF = 575.5 × 10−6), and SiO2(IMF = 57.5 × 10−6). These in vitroresults, obtained from mouse lymphoma cells, support the genotoxic potential of NPs as well as focus thediscussion of the benefits/risks associated with their use in different areas

Source Citation

Demir, E., & Castranova, V. (2016). Genotoxic effects of synthetic amorphous silica nanoparticles in the mouse lymphoma assay. Toxicology Reports, 3, 807–815.


© 2016 Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-NDlicense (



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