Document Type
Article
Publication Date
2014
College/Unit
School of Medicine
Department/Program/Center
Biochemistry
Abstract
We show that the splicing regulator PTBP2 controls a genetic program essential for neuronal maturation. Depletion of PTBP2 in developing mouse cortex leads to degeneration of these tissues over the first three postnatal weeks, a time when the normal cortex expands and develops mature circuits. Cultured Ptbp2−/−neurons exhibit the same initial viability as wild type, with proper neurite outgrowth and marker expression. However, these mutant cells subsequently fail to mature and die after a week in culture. Transcriptome-wide analyses identify many exons that share a pattern of mis-regulation in the mutant brains, where isoforms normally found in adults are precociously expressed in the developing embryo. These transcripts encode proteins affecting neurite growth, pre- and post-synaptic assembly, and synaptic transmission. Our results define a new genetic regulatory program, where PTBP2 acts to temporarily repress expression of adult protein isoforms until the final maturation of the neuron.
Digital Commons Citation
Li, Qin; Zhaeng, Sika; Han, Areum; Lin, Chia-Ho; Stoilov, Peter; Fu, Xiang-Dong; and Black, Douglas L., "The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation" (2014). Faculty & Staff Scholarship. 2599.
https://researchrepository.wvu.edu/faculty_publications/2599
Source Citation
Abstract. (n.d.). eLife Sciences Publications, Ltd. https://doi.org/10.7554/elife.01201.001
Comments
Copyright Li et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.