Document Type
Article
Publication Date
2012
College/Unit
Eberly College of Arts and Sciences
Department/Program/Center
Biology
Abstract
Here we describe the genome of Mesotoga prima MesG1.Ag4.2, the first genome of a mesophilic Thermotogales bacterium. Mesotoga prima was isolated from a polychlorinated biphenyl (PCB)-dechlorinating enrichment culture from Baltimore Harbor sediments. Its 2.97 Mb genome is considerably larger than any previously sequenced Thermotogales genomes, which range between 1.86 and 2.30 Mb. This larger size is due to both higher numbers of protein-coding genes and larger intergenic regions. In particular, the M. prima genome contains more genes for proteins involved in regulatory functions, for instance those involved in regulation of transcription. Together with its closest relative, Kosmotoga olearia, it also encodes different types of proteins involved in environmental and cell–cell interactions as compared with other Thermotogales bacteria. Amino acid composition analysis of M. prima proteins implies that this lineage has inhabited low-temperature environments for a long time. A large fraction of the M. prima genome has been acquired by lateral gene transfer (LGT): a DarkHorse analysis suggests that 766 (32%) of predicted protein-coding genes have been involved in LGT after Mesotogadiverged from the other Thermotogales lineages. A notable example of a lineage-specific LGT event is a reductive dehalogenase gene—a key enzyme in dehalorespiration, indicating M. prima may have a more active role in PCB dechlorination than was previously assumed.
Digital Commons Citation
Zhaxbayeva, Olga; Swithers, Kristen S.; Foght, Julia; Green, Anna G.; Bruce, David; Detter, Chris; Han, Shunsheng; Teshima, Hazuki; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Ivanova, Natalia; Pati, Amrita; Land, Miriam L.; Dlutek, Marlena; Doolittle, W Ford; Noll, Kenneth M.; and Nesbo, Camilla L., "Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date" (2012). Faculty & Staff Scholarship. 2717.
https://researchrepository.wvu.edu/faculty_publications/2717
Source Citation
Zhaxybayeva, O., Swithers, K. S., Foght, J., Green, A. G., Bruce, D., Detter, C., Han, S., Teshima, H., Han, J., Woyke, T., Pitluck, S., Nolan, M., Ivanova, N., Pati, A., Land, M. L., Dlutek, M., Doolittle, W. F., Noll, K. M., & Nesbø, C. L. (2012). Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date. Genome Biology and Evolution, 4(8), 812–820. https://doi.org/10.1093/gbe/evs059
Comments
ß The Author(s) 2012. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.