Document Type
Article
Publication Date
2011
College/Unit
Davis College of Agriculture, Natural Resources and Design
Department/Program/Center
Division of Animal and Nutritional Sciences
Abstract
Background
Rainbow trout (Oncorhynchus mykiss) are the most-widely cultivated cold freshwater fish in the world and an important model species for many research areas. Coupling great interest in this species as a research model with the need for genetic improvement of aquaculture production efficiency traits justifies the continued development of genomics research resources. Many quantitative trait loci (QTL) have been identified for production and life-history traits in rainbow trout. An integrated physical and genetic map is needed to facilitate fine mapping of QTL and the selection of positional candidate genes for incorporation in marker-assisted selection (MAS) programs for improving rainbow trout aquaculture production.
Results
The first generation integrated map of the rainbow trout genome is composed of 238 BAC contigs anchored to chromosomes of the genetic map. It covers more than 10% of the genome across segments from all 29 chromosomes. Anchoring of 203 contigs to chromosomes of the National Center for Cool and Cold Water Aquaculture (NCCCWA) genetic map was achieved through mapping of 288 genetic markers derived from BAC end sequences (BES), screening of the BAC library with previously mapped markers and matching of SNPs with BES reads. In addition, 35 contigs were anchored to linkage groups of the INRA (French National Institute of Agricultural Research) genetic map through markers that were not informative for linkage analysis in the NCCCWA mapping panel. The ratio of physical to genetic linkage distances varied substantially among chromosomes and BAC contigs with an average of 3,033 Kb/cM.
Conclusions
The integrated map described here provides a framework for a robust composite genome map for rainbow trout. This resource is needed for genomic analyses in this research model and economically important species and will facilitate comparative genome mapping with other salmonids and with model fish species. This resource will also facilitate efforts to assemble a whole-genome reference sequence for rainbow trout.
Digital Commons Citation
Palti, Yniv; Genet, Carine; Luo, Ming-Cheng; Gao, Guangtu; Charlet, Aurelie; Hu, Yuqin; Castano-Sanchez, Cecilia; Tabet-Canale, Kamila; Krieg, Francine; Yao, Jianbo; Vallejo, Roger L.; and Rexroad III, Caird E., "A first generation integrated map of the rainbow trout genome" (2011). Faculty & Staff Scholarship. 2741.
https://researchrepository.wvu.edu/faculty_publications/2741
Source Citation
Palti, Y., Genet, C., Luo, M. et al. A first generation integrated map of the rainbow trout genome. BMC Genomics 12, 180 (2011). https://doi.org/10.1186/1471-2164-12-180
Comments
© 2011 Palti et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.