Document Type

Article

Publication Date

2011

College/Unit

Eberly College of Arts and Sciences

Department/Program/Center

Physics and Astronomy

Abstract

We describe experimental results on label free imaging of striated skeletal muscle using second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy. The complementarity of the SHG and CARS data makes it possible to clearly identify the main sarcomere sub-structures such as actin, myosin, acto-myosin, and the intact T-tubular system as it emanates from the sarcolemma. Owing to sub-micron spatial resolution and the high sensitivity of the CARS microscopy technique we were able to resolve individual myofibrils. In addition, key organelles such as mitochondria, cell nuclei and their structural constituents were observed revealing the entire structure of the muscle functional units. There is a noticeable difference in the CARS response of the muscle structure within actin, myosin and t-tubule areas with respect to laser polarization. We attribute this to a preferential alignment of the probed molecular bonds along certain directions. The combined CARS and SHG microscopy approach yields more extensive and complementary information and has a potential to become an indispensable method for live skeletal muscle characterization.

Source Citation

Pfeffer, C. P., Olsen, B. R., Ganikhanov, F., & Légaré, F. (2011). Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy. Biomedical Optics Express, 2(5), 1366. https://doi.org/10.1364/boe.2.001366

Comments

©2011 Optical Society of America

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