Document Type
Article
Publication Date
2018
College/Unit
Davis College of Agriculture, Natural Resources and Design
Department/Program/Center
Agricultural & Extension Education
Abstract
The objective of this study was to isolate and characterize antagonistic rhizobacteria from chili against a notorious phytopathogen Phytophthora capsici. Among the 48 bacteria isolated, BTLbbc-02, BTLbbc-03, and BTLbbc-05 were selected based on their inhibitory activity against P. capsici. They were tentatively identified as Burkholderia metallica BTLbbc-02, Burkholderia cepacia BTLbbc-03, and Pseudomonas aeruginosa BTLbbc-05, respectively, based on their 16S rRNA gene sequencing. All inhibited the growth of P. capsici at varying levels by inducing characteristic morphological alterations of P. capsici hyphae. The cell-free culture supernatant of all three isolates impaired motility (up to 100%) and caused lysis (up to 50%) of the halted zoospores. Bioassays revealed that Pseudomonas sp. had higher antagonism and zoospore motility-inhibitory effects against P. capsici compared with two other isolates, Burkholderia spp. and B. metallica, which caused vacuolation in mycelium. All three bacteria suppressed sporangium formation and zoosporogenesis of P. capsici, and improved the seed germination and growth of cucumber. Our findings suggest that epiphytic bacteria, B. metallica, B. cepacia, and P. aeruginosa, could be used as potential biocontrol agents against P. capsici. A further study is required to ensure conformity with the existing regulations for soil, plant, and human health.
Digital Commons Citation
Khatun, Amena; Farhana, Tarin; Sabir, Abdullah As; Islam, Shah Mohammad Naimul; West, Helen M.; Rahman, Mahfuzur; and Islam, Tofazzal, "Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici" (2018). Faculty & Staff Scholarship. 3036.
https://researchrepository.wvu.edu/faculty_publications/3036
Source Citation
Khatun, A., Farhana, T., Sabir, A., Islam, S., West, H., Rahman, M. & Islam, T. (2018). Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici: . Zeitschrift für Naturforschung C, 73(3-4), 123-135. https://doi.org/10.1515/znc-2017-0065