Authors

Elena E. Tchekneva, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Mounika U.L. Goruganthu, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Roman V. Uzhachenko, Meharry Medical College School of Medicine
Portia L. Thomas, Meharry Medical College School of Medicine
Anneliese Antonucci, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Irina Chekneva, Moscow State Medical University Moscow Russia
Michael Koenig, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Longzhu Piao, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Anwari Akhter, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Maria Teresa P. de Aquino, Meharry Medical College School of Medicine
Paravathi Ranganathan, The Ohio State University Wexner Medical Center
Nicholas Long, The Ohio State University
Thomas Magliery, The Ohio State University
Anna Valujskikh, Cleveland Clinic
Jason V. Evans, West Virginia University
Rajeswara R. Arasada, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Pierre P. Massion, Vanderbilt University
David P. Carbone, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center
Anil Shanker, Meharry Medical College School of Medicine
Mikhail M. Dikov, The Ohio State University Wexner Medical Center and The James Comprehensive Cancer Center

Document Type

Article

Publication Date

2019

College/Unit

School of Medicine

Department/Program/Center

Pathology, Anatomy and Laboratory Medicine

Abstract

Background: Notch intercellular communication instructs tissue-specific T-cell development and function. In this study, we explored the roles of dendritic cell (DC)-expressed Notch ligands in the regulation of T-cell effector function. Methods: We generated mice with CD11c lineage-specific deletion of Notch Delta-like ligand (Dll)1 and Jagged (Jag)2. Using these genetically-ablated mice and engineered pharmacological Notch ligand constructs, the roles of various Delta-like and Jagged ligands in the regulation of T-cell-mediated immunity were investigated. We assessed tumor growth, mouse survival, cytokine production, immunophenotyping of myeloid and lymphoid populations infiltrating the tumors, expression of checkpoint molecules and T-cell function in the experimental settings of murine lung and pancreatic tumors and cardiac allograft rejection. Correlative studies were also performed for the expression of NOTCH ligands, NOTCH receptors and PD-1 on various subsets of myeloid and lymphoid cells in tumor-infiltrating immune cells analyzed from primary human lung cancers. Results: Mice with CD11c lineage-specific deletion of Notch ligand gene Dll1, but not Jag2, exhibited accelerated growth of lung and pancreatic tumors concomitant with decreased antigen-specific CD8+ T-cell functions and effector-memory (Tem) differentiation. Increased IL-4 but decreased IFN-γ production and elevated populations of T-regulatory and myeloid-derived suppressor cells were observed in Dll1-ablated mice. Multivalent clustered DLL1-triggered Notch signaling overcame DC Dll1 deficiency and improved anti-tumor T-cell responses, whereas the pharmacological interference by monomeric soluble DLL1 construct suppressed the rejection of mouse tumors and cardiac allograft. Moreover, monomeric soluble JAG1 treatment reduced T-regulatory cells and improved anti-tumor immune responses by decreasing the expression of PD-1 on CD8+ Tem cells. A significant correlation was observed between DC-expressed Jagged and Delta-like ligands with Tem-expressed PD-1 and Notch receptors, respectively, in human lung tumor-infiltrates.Conclusion: Our data show the importance of specific expression of Notch ligands on DCs in the regulation of Tcell effector function. Thus, strategies incorporating selectively engineered Notch ligands could provide a novel approach of therapeutics for modulating immunity in various immunosuppressive conditions including cancer. Keywords: Delta-like notch ligands, Jagged, Notch receptors, Lung carcinoma, Tumor infiltrating immune cells, Heart allograft rejection, Dendritic cells, CD8 T-cells, Regulatory T-cells, Cancer immunotherapy

Source Citation

Tchekneva, E. E., Goruganthu, M. U. L., Uzhachenko, R. V., Thomas, P. L., Antonucci, A., Chekneva, I., Koenig, M., Piao, L., Akhter, A., de Aquino, M. T. P., Ranganathan, P., Long, N., Magliery, T., Valujskikh, A., Evans, J. V., Arasada, R. R., Massion, P. P., Carbone, D. P., Shanker, A., & Dikov, M. M. (2019). Correction to: Determinant roles of dendritic cell-expressed Notch Delta-like and Jagged ligands on anti-tumor T-cell immunity. Journal for ImmunoTherapy of Cancer, 7(1). https://doi.org/10.1186/s40425-019-0592-2

Comments

© The Author(s). 2019, corrected publication 2019. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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