Author ORCID Identifier

https://orcid.org/0000-0002-9496-6898

https://orcid.org/0000-0003-3698-0234

https://orcid.org/0000-0003-2580-5799

https://orcid.org/0000-0003-0206-5388

Document Type

Article

Publication Date

2019

College/Unit

Eberly College of Arts and Sciences

Department/Program/Center

Biology

Abstract

PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty-eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross-amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well-supported clade. CONCLUSIONS: These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum

Source Citation

Sheik, M. L., K. L. LaBounty, E. Mitchell, and E. L. Gillespie. 2019. Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum). Applications in Plant Sciences 7(12): e11306.

Comments

© 2019 Sheik et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Biology Commons

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