Document Type
Article
Publication Date
2018
College/Unit
School of Pharmacy
Department/Program/Center
Pharmaceutical Sciences
Abstract
Kisspeptin/Neurokinin B/Dynorphin (KNDy) neurons of the arcuate nucleus (ARC) play a key role in the regulation of fertility. The ability to detect features of KNDy neurons that are essential for fertility may require three-dimensional (3D) imaging of the complete population. Recently developed protocols for optical tissue clearing permits 3D imaging of neuronal populations in un-sectioned brains. However, these techniques have largely been described in the mouse brain. We report 3D imaging of the KNDy cell population in the whole rat brain and sheep hypothalamus using immunolabelling and modification of a solvent-based clearing protocol, iDISCO. This study expands the use of optical tissue clearing for multiple mammalian models and provides versatile analysis of KNDy neurons across species. Additionally, we detected a small population of previously unreported kisspeptin neurons in the lateral region of the ovine mediobasal hypothalamus, demonstrating the ability of this technique to detect novel features of the kisspeptin system.
Digital Commons Citation
Moore, Aleisha M.; Lucas, Kathryn A.; Goodman, Robert L.; Coolen, Lique M.; and Lehman, Michael N., "Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry" (2018). Faculty & Staff Scholarship. 1815.
https://researchrepository.wvu.edu/faculty_publications/1815
Source Citation
Moore, A. M., Lucas, K. A., Goodman, R. L., Coolen, L. M., & Lehman, M. N. (2018). Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-20563-2
Included in
Biological and Chemical Physics Commons, Medical Anatomy Commons, Medical Neurobiology Commons, Pharmacy and Pharmaceutical Sciences Commons
Comments
Open Access This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre- ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per- mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2018