Document Type
Article
Publication Date
11-8-2018
Department/Program/Center
Biochemistry
Abstract
An electron paramagnetic resonance (EPR)- based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse models of human-derived pancreatic ductal adenocarcinoma cells. The proposed EPR-based pH mapping method enabled quantitative visualization of regional changes in extracellular pH associated with altered tumor metabolism.
Digital Commons Citation
Komarov, Denis A.; Ichikawa, Yuki; Yamamoto, Kumiko; Stewart, Neil J.; Matsumoto, Shingo; Yasui, Hironobu; Kirilyuk, Igor A.; Khramtsov, Valery V.; Inanami, Osamu; and Hirata, Hiroshi, "In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance" (2018). Clinical and Translational Science Institute. 30.
https://researchrepository.wvu.edu/ctsi/30
Source Citation
Komarov DA, Ichikawa Y, Yamamoto K, et al. In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance. Analytical Chemistry. 2018;90(23):13938-13945. doi:10.1021/acs.analchem.8b03328