Document Type
Article
Publication Date
3-1-2018
College/Unit
School of Medicine
Department/Program/Center
Biochemistry
Abstract
Superoxide radical represents one of the most biologically relevant reactive oxygen species involved in numerous physiological and pathophysiological processes. Superoxide measurement through the decay of an EPR signal of a triarylmethyl (TAM) radical possesses the advantage of a high selectivity and relatively high rate constant of TAM reaction with the superoxide. Hereby we report a straightforward synthesis and characterization of a TAM-TAM biradical showing a high reactivity with superoxide (second-order rate constant, (6.7±0.2)·103 M-1 s-1) enabling the measurement of superoxide radical by following the increase of a sharp EPR signal associated with the formation of a TAM-quinone methide monoradical product.
Digital Commons Citation
Poncelet, Martin; Driesschaert, Benoit; Bobko, Andrey A.; and Khramtsov, Valery V., "Triarylmethyl-based biradical as a superoxide probe" (2018). Clinical and Translational Science Institute. 776.
https://researchrepository.wvu.edu/ctsi/776
Source Citation
Poncelet M, Driesschaert B, Bobko AA, Khramtsov VV. Triarylmethyl-based biradical as a superoxide probe. Free Radical Research. 2017;52(3):373-379. doi:10.1080/10715762.2017.1369058