Date of Graduation
School of Dentistry
John G. Thomas.
Background: The ideal goal of endodontic therapy is to decrease the microbial load within the root canal system followed by the placement of a hermetic seal of the apical portion of the root. Sodium hypochlorite (NaOCl) has long been considered the gold standard for disinfecting root canals, but, the presence of a persistent intraradicular biofilm in properly cleaned and shaped canals has led to the need of development for adjunct irrigant targeting these complex structures. Objectives: The purpose of this study was to compare the effects on the biofilm of NaOCl and delmopinol hydrochloride (DH), a new FDA approved oral rinse, on multiple species biofilms. Methods: Preliminary data was collected with zones of inhibition on Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa grown on Mueller-Hinton vs 30% Poloxamer (biofilm phenotype) plates incubated at 37C for 24 hours. The effect was then studied using a Calgary Bioflm Device with crystal violet staining (CV), scanning electron microscope imaging (SEM) and sonication with Mueller-Hinton plating. Results: The data showed that delmopinol hydrochloride does not possess the strong antimicrobial properties that sodium hypochlorite does. However, delmopinol hydrochloride did possess antibiofilm capabilities, on all organisms that remained, even at diluted concentrations. Conclusion: Delmopinol hydrochloride is not an irrigant to replace sodium hypochlorite, but could be used as an adjunct in the final steps of irrigation to reduce biofilm colonization..
Holley, Gabriel M., "Effect of Delmopinol Hydrochloride on Endodontic Biofilm" (2013). Graduate Theses, Dissertations, and Problem Reports. 3610.