Date of Graduation


Document Type


Degree Type



School of Dentistry



Committee Chair

Matthew S Bryington

Committee Co-Chair

Bryan Dye

Committee Member

Elizabeth Kao


Objectives: The purpose of this pilot study is to determine if, in healthy states, there are significant differences in cytokine activity in peri-implant crevicular fluid (PICF) between implants restored in an edentulous patient (ES) and implants restored in a dentate patient (DS).;Methods: In this pilot study, peri-implant crevicular fluid was obtained from sixteen total patients --eight dentate patients restored with fixed restoration implants and eight edentulous patients restored with implant retained overdenture. Paper points were used to obtained the peri-implant GCF and then were processed using the MSD Meso Quickplex SQ 120 machine. A 96-well Proinflammatory Panel 1 (human) Kit V-Plex, coated with capture-antibodies to IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and, TNF-alpha, and a 96-well Cytokine Panel 1 (Human) kit V-Plex, coated with capture-antibodies to GM-CSF, IL-1alpha, IL-5, IL-7, IL-12/23 p40, IL-15, IL-16, IL-17A, TNF-beta, and, VEGF, were used and prepared exactly according to manufacturer specifications without deviation.;Results: There were significant differences between PICF of ES group and DS groups. Significant differences, at the p<0.05 interval, were noted in IL-1alpha, IL-12/23p40, IL-10, IL-15, and VEGF.;Conclusions: Statistically significant differences were detected between certain cytokines in PICF of dentate subjects compared to edentulous subjects. There was no correlation noted in the numerical concentrations of cytokines between the statistically significant differences. There is a tendency towards higher levels of cytokines/inflammatory markers in PICF of implants in dentate patients compared to edentulous patients.