Title

Evaluation of non-invasive biomarkers for carcinogenic exposure to cigarette smoke

Semester

Spring

Date of Graduation

1999

Document Type

Thesis

Degree Type

MS

College

School of Pharmacy

Department

Pharmaceutical Sciences

Committee Chair

Mohamadi Sarkar

Committee Co-Chair

Timothy Tracy

Committee Member

Ainsley Weston

Committee Member

Ann Berry

Abstract

Purpose. To investigate urinary 1-hydroxypyrene glucuronide (1-OHPG) and benzo (a) pyrene 7,10/8,-tetrol (BaPT) levels as non-invasive biomarkers for exposure to cigarette smoke.;Methods. Volunteers, smokers ({dollar}\ge{dollar}20 cigarettes/day) and non-smokers (5 females + 6 males in each group), provided multiple urine samples. Urine samples from psoriasis patients undergoing coal tar therapy were also obtained. Urinary BaPT and 1-OHPG levels were assayed.;Results. While BaPT levels ranged from undetectable to 11 pmol/10ml in psoriasis patients (mean = 4 {dollar}\pm{dollar} 3.4 pmol/10ml), most samples from smokers or non-smokers had undetectable levels (limit of detection = 25 fmol/10ml urine). Urinary 1-OHPG levels were significantly higher among smokers (mean log 1-OHPG = 1.72 {dollar}\pm{dollar} 0.93) than among nonsmokers (mean log 1-OHPG = 0.80 {dollar}\pm{dollar} 0.92) (P {dollar}<{dollar} 0.05). Conclusion. Higher assay sensitivity is required to monitor urinary levels of BaPT in smokers. 1-OHPG levels in urine may prove useful in monitoring exposure to carcinogenic PAH from tobacco smoke.

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