Document Type
Article
Publication Date
2018
College/Unit
Eberly College of Arts and Sciences
Department/Program/Center
Biochemistry
Abstract
The group II intron and the spliceosome share a common active site architecture and are thought to be evolutionarily related. Here we report the 3.7 Å crystal structure of a eukaryotic group II intron in the lariat-3′ exon form, immediately preceding the second step of splicing, analogous to the spliceosomal P complex. This structure reveals the location of the intact 3′ splice site within the catalytic core of the group II intron. The 3′-OH of the 5′ exon is positioned in close proximity to the 3′ splice site for nucleophilic attack and exon ligation. The active site undergoes conformational rearrangements with the catalytic triplex having dif- ferent configurations before and after the second step of splicing. We describe a complete model for the second step of group II intron splicing that incorporates a dynamic catalytic triplex being responsible for creating the binding pocket for 3′ splice site capture.
Digital Commons Citation
Chan, Russell T.; Peters, Jessica K.; Robart, Aaron R.; Wiryaman, Timothy; Rajashankar, Kanagalaghatta R.; and Toor, Navtej, "Structural basis for the second step of group II intron splicing" (2018). Faculty & Staff Scholarship. 1349.
https://researchrepository.wvu.edu/faculty_publications/1349
Source Citation
Chan, R. T., Peters, J. K., Robart, A. R., Wiryaman, T., Rajashankar, K. R., & Toor, N. (2018). Structural basis for the second step of group II intron splicing. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-06678-0
Comments
Open Access This article is licensed under a Creative Commons
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