Document Type
Article
Publication Date
2019
Department/Program/Center
Psychology
Abstract
Background
Dental caries is the most common chronic disease in the US and disproportionately affects racial/ethnic minorities. Caries is heritable, and though genetic heterogeneity exists between ancestries for a substantial portion of loci associated with complex disease, a genome-wide association study (GWAS) of caries specifically in African Americans has not been performed previously.
Methods
We performed exploratory GWAS of dental caries in 109 African American adults (age > 18) and 96 children (age 3–12) from the Center for Oral Health Research in Appalachia (COHRA1 cohort). Caries phenotypes (DMFS, DMFT, dft, and dfs indices) assessed by dental exams were tested for association with 5 million genotyped or imputed single nucleotide polymorphisms (SNPs), separately in the two age groups. The GWAS was performed using linear regression with adjustment for age, sex, and two principal components of ancestry. A maximum of 1 million adaptive permutations were run to determine empirical significance.
Results
No loci met the threshold for genome-wide significance, though some of the strongest signals were near genes previously implicated in caries such as antimicrobial peptide DEFB1 (rs2515501; p = 4.54 × 10− 6) and TUFT1 (rs11805632; p = 5.15 × 10− 6). Effect estimates of lead SNPs at suggestive loci were compared between African Americans and Caucasians (adults N = 918; children N = 983). Significant (p < 5 × 10− 8) genetic heterogeneity for caries risk was found between racial groups for 50% of the suggestive loci in children, and 12–18% of the suggestive loci in adults.
Conclusions
The genetic heterogeneity results suggest that there may be differences in the contributions of genetic variants to caries across racial groups, and highlight the critical need for the inclusion of minorities in subsequent and larger genetic studies of caries in order to meet the goals of precision medicine and to reduce oral health disparities.
Digital Commons Citation
Orlova, E.; Carlson, J. C.; Lee, M. K.; Feingold, E.; McNeil, D. W.; Crout, R. J.; Weyant, R. J.; Marazita, M. L.; and Shaffer, J. R., "Pilot GWAS of Caries in African-Americans Shows Genetic Heterogeneity" (2019). Faculty & Staff Scholarship. 1688.
https://researchrepository.wvu.edu/faculty_publications/1688
Source Citation
Orlova, E., Carlson, J. C., Lee, M. K., Feingold, E., McNeil, D. W., Crout, R. J., … Shaffer, J. R. (2019). Pilot GWAS of caries in African-Americans shows genetic heterogeneity. BMC Oral Health, 19(1). https://doi.org/10.1186/s12903-019-0904-4
Comments
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.