Theaflavin-3,3′-Digallate Enhances the Inhibitory Effect of Cisplatin by Regulating the Copper Transporter 1 and Glutathione in Human Ovarian Cancer Cells

Authors

Haibo Pan, Zhejiang University & Alderson Broaddus University
Eunhye Kim, Zhejiang University
Gary O. Rankin, Marshall University
Yon Rojanasakul, West Virginia University
Youying Tu, Zhejiang University
Yi Charlie Chen, Alderson Broaddus UniversityFollow

Author ORCID Identifier

N/A

https://orcid.org/0000-0003-2432-3157

https://orcid.org/0000-0002-5385-3512

https://orcid.org/0000-0002-8839-6462

N/A

N/A

Document Type

Article

Publication Date

2018

College/Unit

School of Medicine

Department/Program/Center

Medicine

Abstract

Ovarian cancer has the highest fatality rate among the gynecologic cancers. The side effects, high relapse rate, and drug resistance lead to low long-term survival rate (less than 40%) of patients with advanced ovarian cancer. Theaflavin-3,3′-digallate (TF3), a black tea polyphenol, showed less cytotoxicity to normal ovarian cells than ovarian cancer cells. We aimed to investigate whether TF3 could potentiate the inhibitory effect of cisplatin against human ovarian cancer cell lines. In the present study, combined treatment with TF3 and cisplatin showed a synergistic cytotoxicity against A2780/CP70 and OVCAR3 cells. Treatment with TF3 could increase the intracellular accumulation of platinum (Pt) and DNA-Pt adducts and enhanced DNA damage induced by cisplatin in both cells. Treatment with TF3 decreased the glutathione (GSH) levels and upregulated the protein levels of the copper transporter 1 (CTR1) in both cells, which led to the enhanced sensitivity of both ovarian cancer cells to cisplatin. The results imply that TF3 might be used as an adjuvant to potentiate the inhibitory effect of cisplatin against advanced ovarian cancer.

Digital Commons Citation

Pan, Haibo; Kim, Eunhye; Rankin, Gary O.; Rojanasakul, Yon; Tu, Youying; and Chen, Yi Charlie, "Theaflavin-3,3′-Digallate Enhances the Inhibitory Effect of Cisplatin by Regulating the Copper Transporter 1 and Glutathione in Human Ovarian Cancer Cells" (2018). Faculty & Staff Scholarship. 2093.
https://researchrepository.wvu.edu/faculty_publications/2093

Source Citation

Pan, H., Kim, E., Rankin, G., Rojanasakul, Y., Tu, Y., & Chen, Y. (2018). Theaflavin-3,3′-Digallate Enhances the Inhibitory Effect of Cisplatin by Regulating the Copper Transporter 1 and Glutathione in Human Ovarian Cancer Cells. International Journal of Molecular Sciences, 19(1), 117. https://doi.org/10.3390/ijms19010117

Comments

1. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).