Document Type
Article
Publication Date
2015
College/Unit
Eberly College of Arts and Sciences
Department/Program/Center
Chemistry
Abstract
Focal adhesion kinase (FAK) is a protein tyrosine kinase that is ubiquitously expressed, recruited to focal adhesions, and engages in a variety of cellular signaling pathways. Diverse cellular responses, such as cell migration, proliferation, and survival, are regulated by FAK. Prior to activation, FAK adopts an autoinhibited conformation in which the FERM domain binds the kinase domain, blocking access to the activation loop and substrate binding site. Activation of FAK occurs through conformational change, and acidic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PIP2) are known to facilitate this process. PIP2 binding alters the autoinhibited conformation of the FERM and kinase domains and subsequently exposes the activation loop to phosphorylation. However, the detailed molecular mechanism of PIP2 binding and its role in FAK activation remain unclear. In this study, we conducted coarse-grained molecular dynamics simulations to investigate the binding of FAK to PIP2. Our simulations identified novel areas of basic residues in the kinase domain of FAK that potentially undergo transient binding to PIP2 through electrostatic attractions. Our investigation provides a molecular picture of PIP2-initiated FAK activation and introduces promising new pathways for future studies of FAK regulation.
Digital Commons Citation
Feng, Jun and Mertz, Blake, "Novel Phosphotidylinositol 4,5-Bisphosphate Binding Sites on Focal Adhesion Kinase" (2015). Faculty & Staff Scholarship. 2228.
https://researchrepository.wvu.edu/faculty_publications/2228
Source Citation
Feng J, Mertz B (2015) Novel Phosphotidylinositol 4,5-Bisphosphate Binding Sites on Focal Adhesion Kinase. PLoS ONE 10(7): e0132833. https://doi.org/10.1371/journal.pone.0132833
Comments
© 2015 Feng, Mertz. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited