Document Type
Article
Publication Date
2015
College/Unit
School of Pharmacy
Department/Program/Center
Pharmaceutical Sciences
Abstract
Toxin B is one of the major virulence factors of Clostridium difficile, a bacterium that is responsible for a significant number of diarrhea cases in acute care settings. Due to the prevalence of C. difficile induced diarrhea, rapid and correct diagnosis is crucial in the disease management. In this study, we have employed a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for toxin B. At the end of the 12-round selection, one MRE with high affinity nM) for toxin B was identified. The selected MRE demonstrated low cross binding activities on negative targets: bovine serum albumin, Staphylococcus aureus alpha toxin, Pseudomonas aeruginosaexotoxin A, and cholera toxin of Vibrio cholera. A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.
Digital Commons Citation
Hong, Ka L.; Maher, Eamonn; Williams, Ryan M.; and Sooter, Letha J., "In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter" (2015). Faculty & Staff Scholarship. 2291.
https://researchrepository.wvu.edu/faculty_publications/2291
Source Citation
Hong, K. L., Maher, E., Williams, R. M., & Sooter, L. J. (2015). In VitroSelection of a Single-Stranded DNA Molecular Recognition Element againstClostridium difficileToxin B and Sensitive Detection in Human Fecal Matter. Journal of Nucleic Acids, 2015, 1–12. https://doi.org/10.1155/2015/808495
Comments
Copyright © 2015 Ka Lok Hong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.