Document Type
Article
Publication Date
2016
College/Unit
School of Medicine
Department/Program/Center
Behavioral Medicine and Psychiatry
Abstract
Background. Compared with antidepressant activity of Xiaoyaosan, the role of Xiaoyaosan in anxiety has been poorly studied. Objective. To observe the effects of Xiaoyaosan on anxiety-like behavior induced by chronic immobilization stress (CIS) and further explore whether these effects were related to CRF1R signaling. Methods. Adult male SD rats were randomly assigned to five groups (�� = 12): the nonstressed control group, vehicle-treated (saline, p.o.) group, Xiaoyaosan-treated (3.854 g/kg, p.o.) group, vehicletreated (surgery) group, and antalarmin-treated (surgery) group. Artificial cerebrospinal fluid (0.5 ��L/side) or CRF1R antagonist antalarmin (125 ng/0.5 ��L, 0.5 ��L/side) was bilaterally administered into the basolateral amygdala in the surgery groups. Except for the nonstressed control group, the other four groups were exposed to CIS (14 days, 3 h/day) 30 minutes after treatment. On days 15 and 16, all animals were subjected to the elevated plus-maze (EPM) and novelty suppressed feeding (NSF) test. We then examined the expression of CRF1R, pCREB, and BDNF in the amygdala. Results. Chronic pretreatment with Xiaoyaosan or antalarmin significantly reversed elevated anxiety-like behavior and the upregulated level of CRF1R and BDNF in the amygdala of stressed rats. pCREB did not differ significantly among the groups. Conclusions. These results suggest that Xiaoyaosan exerts anxiolytic-like effects in behavioral tests and the effects may be related to CRF1R signaling in the amygdala
Digital Commons Citation
Jiang, You-Ming; Li, Xiao-Juan; Meng, Zhen-Zhi; Liu, Yue-Yun; Zhao, Hong-Bo; Li, Na; Yan, Zhi-Yi; Ma, Qing-Yu; Zhang, Han-Ting; and Chen, Jia-Xu, "Effects of Xiaoyaosan on Stress-Induced Anxiety-Like Behavior in Rats: Involvement of CRF1 Receptor" (2016). Faculty & Staff Scholarship. 2408.
https://researchrepository.wvu.edu/faculty_publications/2408
Source Citation
Jiang, Y.-M., Li, X.-J., Meng, Z.-Z., Liu, Y.-Y., Zhao, H.-B., Li, N., Yan, Z.-Y., Ma, Q.-Y., Zhang, H.-T., & Chen, J.-X. (2016). Effects of Xiaoyaosan on Stress-Induced Anxiety-Like Behavior in Rats: Involvement of CRF1 Receptor. Evidence-Based Complementary and Alternative Medicine, 2016, 1–9. https://doi.org/10.1155/2016/1238426
Comments
Copyright © 2016 You-Ming Jiang et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.