Toxicity screening of two prevalent metal organic frameworks for therapeutic use in human lung epithelial cells

Authors

Alixandra Wagner, West Virginia University
Qian Liu, West Virginia University
Olivia L. Rose, West Virginia University
Anna Eden, West Virginia University
Aishwarya Vijay, West Virginia University
Yon Rojanasakul, West Virginia University
Cerasela Z. Dinu, West Virginia University

Author ORCID Identifier

N/A

N/A

N/A

https://orcid.org/0000-0001-5131-7081

N/A

N/A

https://orcid.org/0000-0002-6474-6771

Document Type

Article

Publication Date

2019

College/Unit

Statler College of Engineering and Mining Resources

Department/Program/Center

Chemical and Biomedical Engineering

Abstract

Introduction: The flexibility and tunability of metal organic frameworks (MOFs), crystal- line porous materials composed of a network of metal ions coordinated by organic ligands, confer their variety of applications as drug delivery systems or as sensing and imaging agents. However, such properties also add to the difficulty in ensuring their safe implementa- tion when interaction with biological systems is considered.

Methods: In the current study, we used real-time sensorial strategies and cellular-based approaches to allow for fast and effective screening of two MOFs of prevalent use, namely, MIL-160 representative of a hydrophilic and ZIF-8 representative of a hydrophobic frame- work. The two MOFs were synthesized “in house” and exposed to human bronchial epithelial (BEAS-2B) cells, a pertinent toxicological screening model.

Results: Analysis allowed evaluation and differentiation of particle-induced cellular effects as well identification of different degrees and routes of toxicity, all in a high-throughput manner. Our results show the importance of performing screening toxicity assessments before introducing MOFs to biomedical applications.

Discussion: Our proposed screening assays could be extended to a wider variety of cell lines to allow for identification of any deleterious effects of MOFs, with the range of toxic mechanisms to be differentiated based on cell viability, morphology and cell–substrate interactions, respectively.

Conclusion: Our analysis highlights the importance of considering the physicochemical properties of MOFs when recommending a MOF-based therapeutic option or MOFs imple- mentation in biomedical applications.

Digital Commons Citation

Wagner, Alixandra; Liu, Qian; Rose, Olivia L.; Eden, Anna; Vijay, Aishwarya; Rojanasakul, Yon; and Dinu, Cerasela Z., "Toxicity screening of two prevalent metal organic frameworks for therapeutic use in human lung epithelial cells" (2019). Faculty & Staff Scholarship. 2422.
https://researchrepository.wvu.edu/faculty_publications/2422

Source Citation

Wagner, A., Liu, Q., Rose, O. L., Eden, A., Vijay, A., Rojanasakul, Y., & Dinu, C. Z. (2019).

Toxicity screening of two prevalent metal organic frameworks for therapeutic use in human lung epithelial cells

Comments

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