Impairment of Coronary Arteriolar Endothelium-Dependent Dilation after Multi-Walled Carbon Nanotube Inhalation: A Time-Course Study

Authors

Phoebe A. Stapleton, West Virginia University
Valerie C. Minarchick, West Virginia University
Amy M. Cumpston, National Institute for Occupational Safety and Health
Walter McKinney, National Institute for Occupational Safety and Health
Bean T. Chen, National Institute for Occupational Safety and Health
Tina M. Sager, National Institute for Occupational Safety and Health
David G. Frazer, West Virginia University
Robert R. Mercer, National Institute for Occupational Safety and Health
James Scabilloni, National Institute for Occupational Safety and Health
Michael E. Andrew, National Institute for Occupational Safety and Health
Vincent Castranova, National Institute for Occupational Safety and Health
Timothy R. Nurkiewicz, West Virginia University

Author ORCID Identifier

https://orcid.org/0000-0003-3049-4868

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https://orcid.org/0000-0002-5180-8451

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https://orcid.org/0000-0001-9105-970X

Document Type

Article

Publication Date

2013

College/Unit

School of Medicine

Department/Program/Center

Physiology, Pharmacology & Neuroscience

Abstract

Engineered nanomaterials have been developed for widespread applications due to many highly unique and desirable characteristics. The purpose of this study was to assess pulmonary inflammation and subepicardial arteriolar reactivity in response to multi-walled carbon nanotube (MWCNT) inhalation and evaluate the time course of vascular alterations. Rats were exposed to MWCNT aerosols producing pulmonary deposition. Pulmonary inflammation via bronchoalveolar lavage and MWCNT translocation from the lungs to systemic organs was evident 24 h post-inhalation. Coronary arterioles were evaluated 24–168 h post-exposure to determine microvascular response to changes in transmural pressure, endothelium-dependent and -independent reactivity. Myogenic responsiveness, vascular smooth muscle reactivity to nitric oxide, and α-adrenergic responses all remained intact. However, a severe impact on endothelium-dependent dilation was observed within 24 h after MWCNT inhalation, a condition which improved, but did not fully return to control after 168 h. In conclusion, results indicate that MWCNT inhalation not only leads to pulmonary inflammation and cytotoxicity at low lung burdens, but also a low level of particle translocation to systemic organs. MWCNT inhalation also leads to impairments of endothelium-dependent dilation in the coronary microcirculation within 24 h, a condition which does not fully dissipate within 168 h. The innovations within the field of nanotechnology, while exciting and novel, can only reach their full potential if toxicity is first properly assessed.

Digital Commons Citation

Stapleton, Phoebe A.; Minarchick, Valerie C.; Cumpston, Amy M.; McKinney, Walter; Chen, Bean T.; Sager, Tina M.; Frazer, David G.; Mercer, Robert R.; Scabilloni, James; Andrew, Michael E.; Castranova, Vincent; and Nurkiewicz, Timothy R., "Impairment of Coronary Arteriolar Endothelium-Dependent Dilation after Multi-Walled Carbon Nanotube Inhalation: A Time-Course Study" (2013). Faculty & Staff Scholarship. 2649.
https://researchrepository.wvu.edu/faculty_publications/2649

Source Citation

Stapleton, P., Minarchick, V., Cumpston, A., McKinney, W., Chen, B., Sager, T., … Nurkiewicz, T. (2012). Impairment of Coronary Arteriolar Endothelium-Dependent Dilation after Multi-Walled Carbon Nanotube Inhalation: A Time-Course Study. International Journal of Molecular Sciences, 13(12), 13781–13803. https://doi.org/10.3390/ijms131113781

Comments

© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).