Author ORCID Identifier
https://orcid.org/0000-0002-1653-8038
https://orcid.org/0000-0002-2131-7651
N/A
N/A
Document Type
Article
Publication Date
2013
College/Unit
Davis College of Agriculture, Natural Resources and Design
Department/Program/Center
Division of Plant and Soil Sciences
Abstract
Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitroregeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented with 2,4-D and 2-iP. Calli were transferred to MS medium supplemented with 8.8 mM BAP and 0.1 mM NAA (regeneration medium A), or with 4.6 mM kinetin alone (regeneration medium B). The calli were then evaluated for regeneration frequency after sixty days of in vitro cultivation. The noble cultivar 'Jonas' presented the highest rates of plant regeneration among the cultivars tested. The medium A, which contained auxin and cytokinin, induced the highest regeneration rates of all cultivars. The process described herein is simple, reproducible and can potentially be used as a tool in molecular breeding strategies for other marketable cultivars and genotypes of garlic.
Digital Commons Citation
Scotton, Danielle C.; Benedito, Vagner A.; de Molfetta, Jeanne B.; Rodrigues, Benedita I.; Tulmann-Neto, Augusto; and Figueira, Antonio, "Response of root explants to in vitro cultivation of marketable garlic cultivars" (2013). Faculty & Staff Scholarship. 2656.
https://researchrepository.wvu.edu/faculty_publications/2656
Source Citation
Scotton, D. C., Benedito, V. A., Molfetta, J. B. de, Rodrigues, B. I. F., Tulmann-Neto, A., & Figueira, A. (2013). Response of root explants to in vitro cultivation of marketable garlic cultivars. Horticultura Brasileira, 31(1), 80–85. https://doi.org/10.1590/s0102-05362013000100013
Comments
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