Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date

Authors

Olga Zhaxbayeva, West Virginia University
Kristen S. Swithers, University of Connecticut
Julia Foght, University of Alberta
Anna G. Green, University of Connecticut
David Bruce, Los Alamos National Laboratory
Chris Detter, Los Alamos National Laboratory
Shunsheng Han, Los Alamos National Laboratory
Hazuki Teshima, Los Alamos National Laboratory
James Han, DOE Joint Genome Institute
Tanja Woyke, DOE Joint Genome Institute
Sam Pitluck, DOE Joint Genome Institute
Matt Nolan, DOE Joint Genome Institute
Natalia Ivanova, DOE Joint Genome Institute
Amrita Pati, DOE Joint Genome Institute
Miriam L. Land, Oak Ridge National Laboratory
Marlena Dlutek, Dalhousie University
W Ford Doolittle, Dalhousie University
Kenneth M. Noll, University of Connecticut
Camilla L. Nesbo, University of Alberta

Author ORCID Identifier

N/A

N/A

https://orcid.org/0000-0002-8614-3875

https://orcid.org/0000-0001-7548-3682

N/A

N/A

N/A

N/A

https://orcid.org/0009-0002-0333-7568

https://orcid.org/0000-0002-9485-5637

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https://orcid.org/0000-0002-5802-9485

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https://orcid.org/0000-0001-7102-0031

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https://orcid.org/0000-0002-4675-8832

N/A

https://orcid.org/0000-0001-5904-0750

Document Type

Article

Publication Date

2012

College/Unit

Eberly College of Arts and Sciences

Department/Program/Center

Biology

Abstract

Here we describe the genome of Mesotoga prima MesG1.Ag4.2, the first genome of a mesophilic Thermotogales bacterium. Mesotoga prima was isolated from a polychlorinated biphenyl (PCB)-dechlorinating enrichment culture from Baltimore Harbor sediments. Its 2.97 Mb genome is considerably larger than any previously sequenced Thermotogales genomes, which range between 1.86 and 2.30 Mb. This larger size is due to both higher numbers of protein-coding genes and larger intergenic regions. In particular, the M. prima genome contains more genes for proteins involved in regulatory functions, for instance those involved in regulation of transcription. Together with its closest relative, Kosmotoga olearia, it also encodes different types of proteins involved in environmental and cell–cell interactions as compared with other Thermotogales bacteria. Amino acid composition analysis of M. prima proteins implies that this lineage has inhabited low-temperature environments for a long time. A large fraction of the M. prima genome has been acquired by lateral gene transfer (LGT): a DarkHorse analysis suggests that 766 (32%) of predicted protein-coding genes have been involved in LGT after Mesotogadiverged from the other Thermotogales lineages. A notable example of a lineage-specific LGT event is a reductive dehalogenase gene—a key enzyme in dehalorespiration, indicating M. prima may have a more active role in PCB dechlorination than was previously assumed.

Digital Commons Citation

Zhaxbayeva, Olga; Swithers, Kristen S.; Foght, Julia; Green, Anna G.; Bruce, David; Detter, Chris; Han, Shunsheng; Teshima, Hazuki; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Ivanova, Natalia; Pati, Amrita; Land, Miriam L.; Dlutek, Marlena; Doolittle, W Ford; Noll, Kenneth M.; and Nesbo, Camilla L., "Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date" (2012). Faculty & Staff Scholarship. 2717.
https://researchrepository.wvu.edu/faculty_publications/2717

Source Citation

Zhaxybayeva, O., Swithers, K. S., Foght, J., Green, A. G., Bruce, D., Detter, C., Han, S., Teshima, H., Han, J., Woyke, T., Pitluck, S., Nolan, M., Ivanova, N., Pati, A., Land, M. L., Dlutek, M., Doolittle, W. F., Noll, K. M., & Nesbø, C. L. (2012). Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date. Genome Biology and Evolution, 4(8), 812–820. https://doi.org/10.1093/gbe/evs059

Comments

ß The Author(s) 2012. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.