Author ORCID Identifier
N/A
https://orcid.org/0000-0001-6070-231X
Document Type
Article
Publication Date
2011
College/Unit
Eberly College of Arts and Sciences
Department/Program/Center
Physics and Astronomy
Abstract
We describe experimental results on label free imaging of striated skeletal muscle using second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy. The complementarity of the SHG and CARS data makes it possible to clearly identify the main sarcomere sub-structures such as actin, myosin, acto-myosin, and the intact T-tubular system as it emanates from the sarcolemma. Owing to sub-micron spatial resolution and the high sensitivity of the CARS microscopy technique we were able to resolve individual myofibrils. In addition, key organelles such as mitochondria, cell nuclei and their structural constituents were observed revealing the entire structure of the muscle functional units. There is a noticeable difference in the CARS response of the muscle structure within actin, myosin and t-tubule areas with respect to laser polarization. We attribute this to a preferential alignment of the probed molecular bonds along certain directions. The combined CARS and SHG microscopy approach yields more extensive and complementary information and has a potential to become an indispensable method for live skeletal muscle characterization.
Digital Commons Citation
Pfeffer, Christian P.; Olsen, Bjorn R.; Ganikhanov, Feruz; and Legare, Francois, "Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy" (2011). Faculty & Staff Scholarship. 2746.
https://researchrepository.wvu.edu/faculty_publications/2746
Source Citation
Pfeffer, C. P., Olsen, B. R., Ganikhanov, F., & Légaré, F. (2011). Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy. Biomedical Optics Express, 2(5), 1366. https://doi.org/10.1364/boe.2.001366
Comments
©2011 Optical Society of America