Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research

Authors

Rogerio F. Carvalho, Universidade de São Paulo
Marcelo L. Campos, Universidade de São Paulo
Lilian E. Pino, Universidade de São Paulo
Simone L. Crestana, Universidade de São Paulo
Agustin Zsogon, Universidade de São Paulo
Joni E. Lima, Universidade de São Paulo
Vagner A. Benedito, West Virginia University
Lazaro E P Peres, Universidade de São Paulo

Author ORCID Identifier

N/A

https://orcid.org/0000-0001-6235-5120

https://orcid.org/0000-0003-3284-9706

N/A

N/A

https://orcid.org/0000-0001-7828-7425

https://orcid.org/0000-0001-5436-6081

https://orcid.org/0000-0002-2131-7651

https://orcid.org/0000-0002-8761-5934

Document Type

Article

Publication Date

2011

College/Unit

Davis College of Agriculture, Natural Resources and Design

Department/Program/Center

Division of Plant and Soil Sciences

Abstract

Background

The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably compare phenotypes. The lack of tomato developmental mutants in a single genetic background prevents the stacking of mutations to facilitate analysis of double and multiple mutants, often required for elucidating developmental pathways.

Results

We took advantage of the small size and rapid life cycle of the tomato cultivar Micro-Tom (MT) to create near-isogenic lines (NILs) by introgressing a suite of hormonal and photomorphogenetic mutations (altered sensitivity or endogenous levels of auxin, ethylene, abscisic acid, gibberellin, brassinosteroid, and light response) into this genetic background. To demonstrate the usefulness of this collection, we compared developmental traits between the produced NILs. All expected mutant phenotypes were expressed in the NILs. We also created NILs harboring the wild type alleles for dwarf, self-pruning and uniform fruit, which are mutations characteristic of MT. This amplified both the applications of the mutant collection presented here and of MT as a genetic model system.

Conclusions

The community resource presented here is a useful toolkit for plant research, particularly for future studies in plant development, which will require the simultaneous observation of the effect of various hormones, signaling pathways and crosstalk.

Digital Commons Citation

Carvalho, Rogerio F.; Campos, Marcelo L.; Pino, Lilian E.; Crestana, Simone L.; Zsogon, Agustin; Lima, Joni E.; Benedito, Vagner A.; and Peres, Lazaro E P, "Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research" (2011). Faculty & Staff Scholarship. 2754.
https://researchrepository.wvu.edu/faculty_publications/2754

Source Citation

Carvalho, R.F., Campos, M.L., Pino, L.E. et al. Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research. Plant Methods 7, 18 (2011). https://doi.org/10.1186/1746-4811-7-18

Comments

© 2011 Carvalho et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.