Document Type
Article
Publication Date
2011
College/Unit
Eberly College of Arts and Sciences
Department/Program/Center
Microbiology, Immunology, and Cell Biology
Abstract
Background
West Virginia has the worst oral health in the United States, but the reasons for this are unclear. This pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease.
Methods
Bacteria in subgingival plaque samples from twelve participants in two independent West Virginia dental-related studies were characterized using 16S rRNA gene sequencing and Human Oral Microbe Identification Microarray (HOMIM) analysis. Unifrac analysis was used to characterize phylogenetic differences between bacterial communities obtained from plaque of participants with low or high oral disease, which was further evaluated using clustering and Principal Coordinate Analysis.
Results
Statistically different bacterial signatures (P < 0.001) were identified in subgingival plaque of individuals with low or high oral disease in West Virginia based on 16S rRNA gene sequencing. Low disease contained a high frequency of Veillonella and Streptococcus, with a moderate number of Capnocytophaga. High disease exhibited substantially increased bacterial diversity and included a large proportion of Clostridiales cluster bacteria (Selenomonas, Eubacterium, Dialister). Phylogenetic trees constructed using 16S rRNA gene sequencing revealed that Clostridiales were repeated colonizers in plaque associated with high oral disease, providing evidence that the oral environment is somehow influencing the bacterial signature linked to disease.
Conclusions
Culture-independent analyses identified an atypical bacterial signature associated with high oral disease in West Virginians and provided evidence that the oral environment influenced this signature. Both findings provide insight into the etiology of the oral disparity in West Virginia.
Digital Commons Citation
Olson, Joan C.; Cuff, Christopher F.; Lukomski, Slawomir; Lukomska, Ewa; Canizales, Yeremi; Wu, Bei; Crout, Richard J.; Thomas, John G.; McNeil, Daniel W.; Weyant, Robert J.; Marazita, Mary L.; Paster, Bruce J.; and Elliott, Thomas, "Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia" (2011). Faculty & Staff Scholarship. 2790.
https://researchrepository.wvu.edu/faculty_publications/2790
Source Citation
Olson, J.C., Cuff, C.F., Lukomski, S. et al. Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia. BMC Oral Health 11, 7 (2011). https://doi.org/10.1186/1472-6831-11-7
Comments
© 2011 Olson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.