Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): Their expression during early embryonic development

Authors

Raghuveer K. Ramachandra, West Virginia University
Mohamed Salem, West Virginia University
Scott Gahr, National Center for Cool and Cold Water Aquaculture
Caird E. Rexroad III, National Center for Cool and Cold Water Aquaculture
Jianbo Yao, West Virginia University

Author ORCID Identifier

https://orcid.org/0000-0002-3727-3585

https://orcid.org/0000-0003-2142-6716

N/A

https://orcid.org/0000-0002-3683-6147

https://orcid.org/0000-0001-8419-7290

Document Type

Article

Publication Date

2008

College/Unit

Davis College of Agriculture, Natural Resources and Design

Department/Program/Center

Division of Animal and Nutritional Sciences

Abstract

Background

Current literature and our previous results on expression patterns of oocyte-specific genes and transcription factors suggest a global but highly regulated maternal mRNA degradation at the time of embryonic genome activation (EGA). MicroRNAs (miRNAs) are small, non-coding regulatory RNAs (19–23 nucleotides) that regulate gene expression by guiding target mRNA cleavage or translational inhibition. These regulatory RNAs are potentially involved in the degradation of maternally inherited mRNAs during early embryogenesis.

Results

To identify miRNAs that might be important for early embryogenesis in rainbow trout, we constructed a miRNA library from a pool of unfertilized eggs and early stage embryos. Sequence analysis of random clones from the library identified 14 miRNAs, 4 of which are novel to rainbow trout. Real-time PCR was used to measure the expression of all cloned miRNAs during embryonic development. Four distinct expression patterns were observed and some miRNAs showed up-regulated expression during EGA. Analysis of tissue distribution of these miRNAs showed that some are present ubiquitously, while others are differentially expressed among different tissues. We also analyzed the expression patterns of Dicer, the enzyme required for the processing of miRNAs and Stat3, a transcription factor involved in activating the transcription of miR-21. Dicer is abundantly expressed during EGA and Stat3 is up-regulated before the onset of EGA.

Conclusion

This study led to the discovery of 14 rainbow trout miRNAs. Our data support the notion that Dicer processes miRNAs and Stat3 induces expression of miR-21 and possibly other miRNAs during EGA. These miRNAs in turn guide maternal mRNAs for degradation, which is required for normal embryonic development.

Digital Commons Citation

Ramachandra, Raghuveer K.; Salem, Mohamed; Gahr, Scott; Rexroad III, Caird E.; and Yao, Jianbo, "Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): Their expression during early embryonic development" (2008). Faculty & Staff Scholarship. 2853.
https://researchrepository.wvu.edu/faculty_publications/2853

Source Citation

Ramachandra, R.K., Salem, M., Gahr, S. et al. Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): Their expression during early embryonic development. BMC Dev Biol 8, 41 (2008). https://doi.org/10.1186/1471-213X-8-41

Comments

© 2008 Ramachandra et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.