Document Type
Article
Publication Date
2009
College/Unit
School of Medicine
Department/Program/Center
Pathology, Anatomy and Laboratory Medicine
Abstract
Background
Engineered iron nanoparticles are being explored for the development of biomedical applications and many other industry purposes. However, to date little is known concerning the precise mechanisms of translocation of iron nanoparticles into targeted tissues and organs from blood circulation, as well as the underlying implications of potential harmful health effects in human.
Results
The confocal microscopy imaging analysis demonstrates that exposure to engineered iron nanoparticles induces an increase in cell permeability in human microvascular endothelial cells. Our studies further reveal iron nanoparticles enhance the permeability through the production of reactive oxygen species (ROS) and the stabilization of microtubules. We also showed Akt/GSK-3β signaling pathways are involved in iron nanoparticle-induced cell permeability. The inhibition of ROS demonstrate ROS play a major role in regulating Akt/GSK-3β – mediated cell permeability upon iron nanoparticle exposure. These results provide new insights into the bioreactivity of engineered iron nanoparticles which can inform potential applications in medical imaging or drug delivery.
Conclusion
Our results indicate that exposure to iron nanoparticles induces an increase in endothelial cell permeability through ROS oxidative stress-modulated microtubule remodeling. The findings from this study provide new understandings on the effects of nanoparticles on vascular transport of macromolecules and drugs.
Digital Commons Citation
Apopa, Patrick L.; Qian, Yong; Shao, Rong; Guo, Nancy L.; Schwegler-Berry, Diane; Pacurari, Maricica; Porter, Dale; Shi, Xianglin; Vallyathan, Val; Castranova, Vincent; and Flynn, Daniel C., "Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling" (2009). Faculty & Staff Scholarship. 2861.
https://researchrepository.wvu.edu/faculty_publications/2861
Source Citation
Apopa, P.L., Qian, Y., Shao, R. et al. Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling. Part Fibre Toxicol 6, 1 (2009). https://doi.org/10.1186/1743-8977-6-1
Comments
© 2009 Apopa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.