Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici

Authors

Amena Khatun, Bangabandhu Sheikh Mujibur Rahman Agricultural University
Tarin Farhana, Bangabandhu Sheikh Mujibur Rahman Agricultural University
Abdullah As Sabir, Bangabandhu Sheikh Mujibur Rahman Agricultural University
Shah Mohammad Naimul Islam, Bangabandhu Sheikh Mujibur Rahman Agricultural University
Helen M. West, University of Nottingham
Mahfuzur Rahman, West Virginia University
Tofazzal Islam, Bangabandhu Sheikh Mujibur Rahman Agricultural University

Author ORCID Identifier

N/A

N/A

N/A

https://orcid.org/0000-0003-1305-1790

N/A

N/A

https://orcid.org/0000-0002-7613-0261

Document Type

Article

Publication Date

2018

College/Unit

Davis College of Agriculture, Natural Resources and Design

Department/Program/Center

Agricultural & Extension Education

Abstract

The objective of this study was to isolate and characterize antagonistic rhizobacteria from chili against a notorious phytopathogen Phytophthora capsici. Among the 48 bacteria isolated, BTLbbc-02, BTLbbc-03, and BTLbbc-05 were selected based on their inhibitory activity against P. capsici. They were tentatively identified as Burkholderia metallica BTLbbc-02, Burkholderia cepacia BTLbbc-03, and Pseudomonas aeruginosa BTLbbc-05, respectively, based on their 16S rRNA gene sequencing. All inhibited the growth of P. capsici at varying levels by inducing characteristic morphological alterations of P. capsici hyphae. The cell-free culture supernatant of all three isolates impaired motility (up to 100%) and caused lysis (up to 50%) of the halted zoospores. Bioassays revealed that Pseudomonas sp. had higher antagonism and zoospore motility-inhibitory effects against P. capsici compared with two other isolates, Burkholderia spp. and B. metallica, which caused vacuolation in mycelium. All three bacteria suppressed sporangium formation and zoosporogenesis of P. capsici, and improved the seed germination and growth of cucumber. Our findings suggest that epiphytic bacteria, B. metallica, B. cepacia, and P. aeruginosa, could be used as potential biocontrol agents against P. capsici. A further study is required to ensure conformity with the existing regulations for soil, plant, and human health.

Digital Commons Citation

Khatun, Amena; Farhana, Tarin; Sabir, Abdullah As; Islam, Shah Mohammad Naimul; West, Helen M.; Rahman, Mahfuzur; and Islam, Tofazzal, "Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici" (2018). Faculty & Staff Scholarship. 3036.
https://researchrepository.wvu.edu/faculty_publications/3036

Source Citation

Khatun, A., Farhana, T., Sabir, A., Islam, S., West, H., Rahman, M. & Islam, T. (2018). Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici: . Zeitschrift für Naturforschung C, 73(3-4), 123-135. https://doi.org/10.1515/znc-2017-0065