Date of Graduation

1998

Document Type

Thesis

Degree Type

MS

College

Eberly College of Arts and Sciences

Department

Chemistry

Committee Chair

Vincent T. Remcho

Abstract

Electrochromatography (CEC), a hybrid of LC and CE, is a recent and fairly unexplored method of analysis, but has already been shown to be a very efficient separation technique. This thesis comprises a study of the Knox-Parcher effect and its influence upon CEC. The Knox-Parcher effect, which has been established in GC and LC, describes the dependence of reduced plate height, h, on the ratio, r, established by the ratio of the channel diameter (dc) to the particle diameter (dp). This study was conducted using particles with a diameter of 60 mm and capillaries having inner diameters ranging from 246 mm to 530 mm. The work herein demonstrates that reduced plate height, h, in CEC is dependent upon such a ratio. An interesting point found was that the sharp increase in h occurred at a lower value of r (~ 4.5) as compared to that of LC (~7). Reasoning for such a change is discussed. The physical arrangement of the packed particles was also examined as a means of explaining the observed phenomenon. A second focal point of this thesis was to explore possible avenues of capillary preparation; various approaches to column preparation are reviewed in this text.

Download

Share

COinS