Date of Graduation
2000
Document Type
Thesis
Degree Type
MS
Committee Chair
Kenneth P. Blemings
Abstract
To understand tissue participation in lysine oxidation, chicken lysine α-ketoglutarate reductase (LKR) activity and lysine oxidation (LOX) were measured in vitro. LKR activity was measured using a spectrophotometer and LOX was measured by collection of 14CO2 from [U-14C] L-lysine. LKR and LOX were measured in homogenates prepared from liver, kidney, pancreas, heart, brain, lungs, spleen, muscle, and intestine. LKR activities were 1000-fold LOX. LKR activity was highest in liver and intestine each approximately 63 µmoles/(hr*g tissue). Activity in other tissues was significantly greater than zero except for muscle. Muscle, liver, and intestine contributed about 70%, 15% and 6.5% respectively of the total oxidative capacity. The Km and Vmax for LKR were 6.96 ± 1.87 mM and 29.4 ± 2.94 µmoles/(hr*g liver) respectively. A significant (p< 0.05) increase in LKR activity was found in lysine adequate (1.1, 2.1, or 3.1%) diets compared to a lysine deficient diet (0.69%).
Recommended Citation
Manangi, Megharaja Kandagal, "Chicken lysine α-ketoglutarate reductase (LKR) in different tissues and effects of graded levels of dietary lysine on LKR and lysine oxidation." (2000). Graduate Theses, Dissertations, and Problem Reports. 10527.
https://researchrepository.wvu.edu/etd/10527