Date of Graduation
2001
Document Type
Thesis
Degree Type
MS
Committee Chair
Walter Kaczmarczyk
Abstract
Amniocentesis identified a fetus with trisomy 8 in one third of metaphase cells while remaining cells were normal. The purpose of this project was to determine the level of trisomy 8 mosaicism within embryonic and extra-embryonic tissues and cell cycle division rates for the two cell lines. Tissue samples collected at birth included cord blood, cord tissue, peripheral blood, and placental tissues. The level of trisomy 8 and normal cells was determined in G-banded metaphases and fluorescence in situ hybridization of interphase cells. Trisomy 8 metaphase and interphase cell investigations identified a significant decrease of trisomy 8 cells undergoing mitosis. Cell cycle kinetics was measured using incorporation of bromodeoxyuridine during 48 hours to determine the number of cell divisions in metaphase cells. There was no evidence suggesting a significant difference in growth rates using Chi-squared goodness of fit test.
Recommended Citation
Pettit, Bonnie J., "Trisomy 8 mosaicism: Cell cycle kinetics and distribution of trisomy 8 and normal cells in embryonic and extra-embryonic tissues." (2001). Graduate Theses, Dissertations, and Problem Reports (ETD). 10592.
https://researchrepository.wvu.edu/etd/10592