Date of Graduation

2002

Document Type

Thesis

Degree Type

MS

Committee Chair

B. Jean Meade

Abstract

An increase in the incidence of respiratory disease reported in a herbicide producing factory correlates with potential exposure to 3-Amino-5-mercapto-1,2,4-triazole (AMT) and AMT based product 1 (AMT-bp1). In animal studies, no signs of systemic toxicity or irritancy were observed with concentrations up to 25% AMT or 40% AMT-bp1. AMT-bp1 was negative while a dose dependent increase was observed following exposure to AMT in the LLNA. An increase in B220+IgE+ cells was observed at 25% AMT exposure. AMT was negative in the MEST (up to 25%). Following dermal exposure 5 days a week, animals exposed to 25% AMT demonstrated elevated levels of total serum IgE and an increase in airway hyperreactivity upon methacholine challenge. These studies indicate that AMT-bp1 does not induce sensitization following dermal exposure; however AMT was identified as a sensitizer and has the potential to induce airway hyperreactivity. These studies were supported in part by NIEHS IAG #Y1-ES0049-03.

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