Semester

Summer

Date of Graduation

2022

Document Type

Thesis

Degree Type

MS

College

School of Dentistry

Department

Not Listed

Committee Chair

Fotinos Panagakos

Committee Co-Chair

Samuel Dorn

Committee Member

Samuel Dorn

Committee Member

Jerry Bouquot

Abstract

Purpose: The aim of this study is to investigate if hypochlorous acid (Vashe and Endocyn), Ozone, sodium hypochlorite (0.5% NaOCl), chlorhexidine (0.12% CHX), and MTAD can be used as a disinfectant for endodontic surgery without causing bone tissue toxicity by comparing their cytotoxic effects to saline on osteoblasts in vitro.

Methods: Human Fetal Osteoblast Cells (hFOB 1.19, ATCC CRL-11372) were used. The cells viability after 10-minute exposure of Vashe, Endocyn, Ozone, NaOCl (0.5%), CHX (0.12%), MTAD, and saline at concentrations of 10, 30, 60, 90 % volume ratio were measured using CCK8 analysis and the absorbance were measured at 450 nm. Saline was used as a negative control. The morphology changes of the cells after exposure of the testing solutions were compared to the negative media control group using a digital inverted microscope.

Results: CCK8 analysis showed 0.5% NaOCl, CHX, MTAD displayed statistically significant cytotoxic effects to osteoblasts as low as 10% concentration compared to saline. Vashe exhibited a concentration dependent cytotoxic effect on osteoblasts. Vashe at 60% and 90% concentration demonstrated statistically significant cytotoxic effects. Endocyn and Ozone at concentrations of 10%, 30%, 60% were not cytotoxic, however, at concentrations of 90%, Endocyn and Ozone produced statistically significant cytotoxic effects. The morphology of cells did not show changes in 10%, 30 % Vashe, 10%, 30% Endocyn, 10%, 30%, 60% Ozone, and all concentration of saline compared to the negative control- media. Morphology of cells were altered after exposure of 10% of 0.5% NaOCl, CHX, and MTAD.

Conclusion: This study concluded that 0.5% NaOCl, CHX, and MTAD had a significant cytotoxic effect on osteoblasts at a concentration as low as 10%. They should not be considered as irrigants for endodontic surgery. Vashe, Endocyn less than 30%, and Ozone less than 60% were not cytotoxic to osteoblasts; and they may be considered as irrigants for endodontic surgery, following further research. Saline produced non-cytotoxic effects to osteoblast at all concentrations.

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