"Cytotoxic Effects of Potential Irrigants for Endodontic Surgery" by Kaiying Haggard

Semester

Summer

Date of Graduation

2022

Document Type

Thesis

Degree Type

MS

College

School of Dentistry

Department

Not Listed

Committee Chair

Fotinos Panagakos

Committee Co-Chair

Samuel Dorn

Committee Member

Samuel Dorn

Committee Member

Jerry Bouquot

Abstract

Purpose: The aim of this study is to investigate if hypochlorous acid (Vashe and Endocyn), Ozone, sodium hypochlorite (0.5% NaOCl), chlorhexidine (0.12% CHX), and MTAD can be used as a disinfectant for endodontic surgery without causing bone tissue toxicity by comparing their cytotoxic effects to saline on osteoblasts in vitro.

Methods: Human Fetal Osteoblast Cells (hFOB 1.19, ATCC CRL-11372) were used. The cells viability after 10-minute exposure of Vashe, Endocyn, Ozone, NaOCl (0.5%), CHX (0.12%), MTAD, and saline at concentrations of 10, 30, 60, 90 % volume ratio were measured using CCK8 analysis and the absorbance were measured at 450 nm. Saline was used as a negative control. The morphology changes of the cells after exposure of the testing solutions were compared to the negative media control group using a digital inverted microscope.

Results: CCK8 analysis showed 0.5% NaOCl, CHX, MTAD displayed statistically significant cytotoxic effects to osteoblasts as low as 10% concentration compared to saline. Vashe exhibited a concentration dependent cytotoxic effect on osteoblasts. Vashe at 60% and 90% concentration demonstrated statistically significant cytotoxic effects. Endocyn and Ozone at concentrations of 10%, 30%, 60% were not cytotoxic, however, at concentrations of 90%, Endocyn and Ozone produced statistically significant cytotoxic effects. The morphology of cells did not show changes in 10%, 30 % Vashe, 10%, 30% Endocyn, 10%, 30%, 60% Ozone, and all concentration of saline compared to the negative control- media. Morphology of cells were altered after exposure of 10% of 0.5% NaOCl, CHX, and MTAD.

Conclusion: This study concluded that 0.5% NaOCl, CHX, and MTAD had a significant cytotoxic effect on osteoblasts at a concentration as low as 10%. They should not be considered as irrigants for endodontic surgery. Vashe, Endocyn less than 30%, and Ozone less than 60% were not cytotoxic to osteoblasts; and they may be considered as irrigants for endodontic surgery, following further research. Saline produced non-cytotoxic effects to osteoblast at all concentrations.

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