Date of Graduation
2002
Document Type
Thesis
Degree Type
MS
College
Eberly College of Arts and Sciences
Department
Not Listed
Committee Chair
Lisa M. Salati
Committee Co-Chair
Peter Mathers
Committee Member
Diana Beattie
Committee Member
Richard Dey
Abstract
HepG2 cells were used as a model system to study the regulation of glucose-6- phosphate dehydrogenase (G6PD). Regulation of hepatic G6PD expression involves changes in the amount of G6PD pre-mRNA in the nucleus in the absence of changes in the transcription rate of the gene. G6PD expression is increased in HepG2 cells incubated with increasing glucose concentrations. G6PD mRNA is increased 3- to 4- fold by incubation in 25 mM glucose, as compared to 0 mM glucose. To determine the cis-acting element involved in this regulation, HepG2 cells were stably transfected with G6PD genomic DNA composed of exons 7 through 13 and surrounding intronic DNA. Expression of this transgene was driven by the CMV promoter. Incubation with glucose resulted in a 3- to 4- fold increase in the amount of transgene mRNA. The future goal is to understand the molecular details of G6PD regulation by glucose in order to provide additional information into new mechanisms of post-transcriptional regulation.
Recommended Citation
Griffith, Brian Nelson, "The study of glucose-6-phosphate dehydrogenase (G6PD) gene regulation in HepG2 cells by glucose induction and the study of G6PD mRNA localization by fluorescent in situ hybridization (FISH)." (2002). Graduate Theses, Dissertations, and Problem Reports. 12911.
https://researchrepository.wvu.edu/etd/12911