"Isolation and preparation of naturally occurring aluminum ligands usin" by Carson Baldwin

Semester

Fall

Date of Graduation

2005

Document Type

Thesis

Degree Type

MS

College

Eberly College of Arts and Sciences

Department

Chemistry

Committee Chair

Aaron T. Timperman.

Abstract

Typically, Al3+ is bound to minerals, but its toxicity towards vascular plants increases with increasing soil acidity. Ectomycorrhizal fungi often confer Al3+ resistance to host plants by unknown mechanisms. The objective of this research was to develop a method for the successful separation of Al3+ binding fungal exudates produced by the fungus Pisolithus tinctorius. A novel Al3+ immobilized metal affinity chromatography (IMAC) technique was developed to extract exudates involved in Al3+ binding. Recovery of exudates from the IMAC column was dependent on elutor type and pH, with ammonium hydroxide being most effective. Exudates exhibited strong binding with Al3+, allowing their selective enrichment and collection. Because IMAC strongly binds Al3+ that forms an Al3+-ligand complex, isolation of Al3+ ligands was possible. Exudates are also separated using reversed phase in order to differentiate between closely related compounds. Utilizing these techniques results in a simpler sample to be characterized with mass spectrometry.

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