Semester
Summer
Date of Graduation
2006
Document Type
Dissertation
Degree Type
PhD
College
Eberly College of Arts and Sciences
Department
Chemistry
Committee Chair
Aaron T. Timperman.
Abstract
We have developed functional components for a comprehensive microfluidic system for proteomic analyses. This dissertation presents development of an electroomsotic pump for delivery of fluids to other microfluidic channels, a hydrodynamic flow restrictor for combining electrokinetic and hydrodynamic flow on a microfluidic chip-ESI-MS interface, and surface coatings to prevent analyte adsorption to microfluidic channels. A microfluidic chip ESI-MS interface with a novel voltage switching component is also developed for the separation of negative analytes at high pH, with positive ion mode ESI-MS detection of the analyte, converted to positive ions via a make-up solution induced pH change.
Recommended Citation
Razunguzwa, Trust T., "Development of microfluidic devices for proteomics" (2006). Graduate Theses, Dissertations, and Problem Reports. 2419.
https://researchrepository.wvu.edu/etd/2419