Semester
Summer
Date of Graduation
2008
Document Type
Thesis
Degree Type
MS
College
Eberly College of Arts and Sciences
Department
Biology
Abstract
The goals of my research project were to clone the E(var)3-5 gene and characterize the lethality of E(var)3-5 mutations. The E(var)3-5 gene was identified by dominant mutations that enhanced PEV of wm4, thus implicating the gene product in promoting a euchromatic chromatin structure (Dorn et al. 1993). These mutations were found to be recessive lethal as well and the lethal phenotype was used to map the E(var)3-5 gene (Dorn et al. 1993; this work). I characterized the lethality of E(var)3-5 mutations and found that lethality in E(var)3-5 hemizygotes is manifested at the pupal stage. I mapped the lethality to a region defined by Df(3R)Exel7310. I generated five FLP-FRT mediated deletions within the region defined by Df(3R)Exel7310 however, my complementation analysis revealed that E(var)3-5 mutants were not allelic to any of the genes in that region. However I identified a mutation in one of the genes, CG17360 in all E(var)3-5 mutants examined. The CG17360 mutants exhibit an unexpanded wing phenotype.
Recommended Citation
Sahasrabhojane, Pranoti, "Identification and characterization of the E(var)3-5 gene in Drosophila melanogaster" (2008). Graduate Theses, Dissertations, and Problem Reports (ETD). 4419.
https://researchrepository.wvu.edu/etd/4419