Semester
Fall
Date of Graduation
2019
Document Type
Dissertation
Degree Type
PhD
College
Eberly College of Arts and Sciences
Department
Biology
Committee Chair
Jennifer Gallagher
Committee Co-Chair
Stephen DiFazio
Committee Member
Stephen DiFazio
Committee Member
Jennifer Hawkins
Committee Member
Timothy Driscoll
Committee Member
Daniel Panaccione
Abstract
Commercial formulations of glyphosate are among the most extensively used herbicides around the world. The active ingredient, glyphosate, targets the aromatic amino acid pathway. This pathway is absent in mammals, resulting in low toxicity. Different formulations contain varying adjuvants and surfactants, whose synergistic effects are yet to be extensively studied at the cellular level. In this study, I tested multiple commercial formulations that showed a variation in growth phenotype among different yeast strains. To gain a better understanding of response and resistance mechanisms at the genome and transcriptome level, I carried out an in-lab evolution study, along with a transcriptome analysis on exposure to a commercial formulation of glyphosate. Overall, my findings helped identify routes of transport of glyphosate in and out of the cell. The additives in these herbicides were found to have effects on the cell wall, cell cycle regulation, transposable elements, and mitochondrial function.
Recommended Citation
Ravi Shankar, Apoorva, "Effects Of Commercial Formulations Of Glyphosate On Saccharomyces Cerevisiae" (2019). Graduate Theses, Dissertations, and Problem Reports. 7458.
https://researchrepository.wvu.edu/etd/7458
Embargo Reason
Publication Pending
Included in
Biology Commons, Genomics Commons, Molecular Genetics Commons