Cytogenetic analyses of clastogens and aneuploidogens by immunofluorescent and micronucleus methods.
Date of Graduation
1990
Document Type
Dissertation
Degree Type
PhD
Committee Chair
W-Z. Whong
Committee Co-Chair
E.C. Keller
Committee Member
W. Kaczmarczyk
Committee Member
T. Ong
Committee Member
J. Nath
Abstract
The cytogenetic effect of various genotoxic compounds was analysed in cytokinesis-blocked human peripheral lymphocytes and V 79 cells by using immunofluorescence and micronucleus methods. Cytochalasin-B (CYB) induced a maximum number of binucleated cells at 3-4 {dollar}\\mu{dollar}g/ml without any genotoxic effect. Comparative studies between binucleated (CB) and mononucleated micronucleus assays show that the micronucleus (MN) frequency in CB method was higher than those in the mononucleated cells (P {dollar}
Recommended Citation
Channarayappa, "Cytogenetic analyses of clastogens and aneuploidogens by immunofluorescent and micronucleus methods." (1990). Graduate Theses, Dissertations, and Problem Reports. 8606.
https://researchrepository.wvu.edu/etd/8606