Activation of Adenosine A2A but not A2B Receptors is Involved in Uridine Adenosine Tetraphosphate-induced Porcine Coronary Smooth Muscle Relaxation

Authors

Changyan Sun, West Virginia University
Tong Jiao, Karolinska University Hospital, Karolinska Institutet
Daphne Merkus, Erasmus University Medical Center, University Hostpital, LMU Munich
Dirk J. Duncker, Erasmus University Medical Center
S. Jamal Mustafa, West Virginia University
Zhichao Zhou, West Virginia University, Karolinska University Hospital, Karolinska Institutet,

Author ORCID Identifier

N/A

N/A

https://orcid.org/0000-0002-4852-831X

N/A

N/A

https://orcid.org/0000-0002-5107-6529

Document Type

Article

Publication Date

2019

Department/Program/Center

Physiology, Pharmacology & Neuroscience

Abstract

Activation of both adenosine A2A and A2B receptors (A2BR) contributes to coronary vasodilation. We previously demonstrated that uridine adenosine tetraphosphate (Up4A) is a novel vasodilator in the porcine coronary microcirculation, acting mainly on A2AR in smooth muscle cells (SMC). We further investigated whether activation of A2BR is involved in Up4A-mediated coronary SMC relaxation. Both A2AR and A2BR may stimulate H2O2 production leading to activation of KATP channels in SMCs, we also studied the involvement of H2O2 and KATP channels in Up4A-mediated effect. Coronary small arteries dissected from the apex of porcine hearts were mounted on wire myograph for Up4A concentration responses. Up4A-induced coronary SMC relaxation was attenuated by A2AR but not A2BR antagonism or non-selective P2R antagonism, despite greater endogenous A2BR expression vs. A2AR in both coronary small arteries and primary cultured coronary SMCs. Moreover, Up4A-induced coronary SMC relaxation was blunted by H2O2 catabolism. This effect was not altered by KATP channel blockade. Combination of H2O2 catabolism and A2AR antagonism attenuated Up4A-induced coronary SMC relaxation to the similar extent as A2AR antagonism alone. Collectively, Up4A-induced porcine coronary SMC relaxation is mediated by activation of A2AR-H2O2 pathway. This process does not involve A2BR, P2R or KATP channels.

Digital Commons Citation

Sun, Changyan; Jiao, Tong; Merkus, Daphne; Duncker, Dirk J.; Mustafa, S. Jamal; and Zhou, Zhichao, "Activation of Adenosine A2A but not A2B Receptors is Involved in Uridine Adenosine Tetraphosphate-induced Porcine Coronary Smooth Muscle Relaxation" (2019). Faculty & Staff Scholarship. 1810.
https://researchrepository.wvu.edu/faculty_publications/1810

Source Citation

Sun, C., Jiao, T., Merkus, D., Duncker, D. J., Mustafa, S. J., & Zhou, Z. (2019). Activation of adenosine A2A but not A2B receptors is involved in uridine adenosine tetraphosphate-induced porcine coronary smooth muscle relaxation. Journal of Pharmacological Sciences, 141(1), 64–69. https://doi.org/10.1016/j.jphs.2019.09.006

Comments

© 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.